Cerno develops and publishes award-winning mass spectrometry calibration and analysis software which not only enables formula determination at unit mass resolution (single or triple quad) with 100x improvement in mass accuracy (CLIPS), a capability typically reserved only for HiRes MS systems, but also improves formula ID at HiRes by 20x through the Spectral Accuracy concept (sCLIPS). These dramatic results are enabled through the patented TrueCal™ calibration process and validated by hundreds of publications and thousands of satisfied users across the world.

For unit resolution quadrupoles:

100x better mass accuracy
up to 99.9% Spectral Accuracy
Confident elemental composition determination
Mixture deconvolution for isotope analysis and large molecule deconvolution

For high resolution TOF, Orbitrap, FT ICR:

Self-calibrates peak shape without standards for exact isotope modeling
Eliminates 95-99% of incorrect formulas with high spectral accuracy
Quantitative measurements for biologics degradation analysis

MassWorks TrueCal™ calibration technology provides advantages for both high and low resolution instruments. The above left figure shows an almost perfect match (Spectral Accuracy) between a calibrated single quad and the corresponding “True” calculated MS using the CLIPS search. The figure on the right shows excellent spectral accuracy match for confident formula ID on a high resolution TOF instrument using sCLIPS search.

Proper mass spec calibration also means that complex mixture deconvolution of overlapping and interfering ions is easy and provides the most accurate quantitative results. The top figure shows the accurate quantitative analysis of 7 component mixture of a C13 radio labeled pharmacutical using a quadrupole instrument. The bottom figure shows the ability to detect and deconvolve modified products (glycan conjugates 0 – 10) of a large molecule intact protein (by Maldi) (see ASMS poster). This type of analysis is not possible by other means!

Powerful GC/MS Analysis Software

GC/ID is the first major breakthrough in GC/MS in decades

GC/ID is a fully automated compound identification application that offers new levels of accuracy and confidence in GC/MS qualitative and semi-quantitative analysis. Based on the new MassWorks Rx configurable platform, GC/ID offers powerful new capabilities to GC/MS including:

Uses the industry standard NIST search engine
Retention Index (RI) matching provides unsurpassed unknown ID confidence
Accurate mass analysis of molecular/fragment ions for confident compound ID
Magic Highlighter indicates at a glance the correct match by combining search, RI, and formula ID

Learn more about GC/ID here.

The software that started it all…

Easy-to-use post acquisition software | Achieve high Mass Accuracy and high Spectral Accuracy

With over a dozen patents and 1000’s of users, the award winning MassWorks established the performance benchmark for “True” mass spec calibration. MassWorks is a Windows based desktop software that works with most MS vendor instruments by directly reading the data files making the powerful calibration and analysis technologies available to both nominal and high resolution instruments. Learn more about it here.

Cerno is an authorized distributor of NIST and Wiley MS libraries and MS software

Get your MS libraries for use with MassWorks Rx GC/ID or stand alone for any MS system. In addition to EI data libraries are available for MS/MS instruments. Most libraries also include extensive meta data including retention time index (used automatically by GC/ID), and chemical structures.

Cerno Introduces SAMMI

Cerno calibration technology provides a new innovative approach to large molecule analysis for peptides, proteins, oligonucleotides, and any multiply charged molecule. Read more about SAMMI.

New! GC/ID™ 4.0 !

Riding on the success of V3 with groundbreaking features including Auto RI™ and Magic Highlighter™, V4 adds revolutionary SMD peak deconvolution along with a host of improvements and upgrades. To learn more about GC/ID check out the brochure or watch the March 22 Webinar.

Watch C&EN Webinar on GC/MS Automated Retention Index

MassWorks Version 6.1 has been released!
Read all About it.

Cerno is an authorized distributor of all NIST and Wiley MS libraries making over 1,000,000 unique compounds available at great prices! Contact us for a quote for new libraries or upgrades. New! NIST23 is now available in Agilent PBM format.

View our recent mini-webinar Series:

Mass Spectral Accuracy: What is it, and Why is it so Important?

Improving and Automating GC/MS Compound ID on Quadruple MS using Accurate Mass, Spectral Accuracy, Retention Index, and Conventional Search

Differentiating the Loss of 43Da EI Fragments (C3H7 or CH3C=O) with Single Quad GC/MS

User Testimonials

"The program is very easy to learn, it literally took minutes to get the first exciting results. On our 10 year-old triple quad, we manage to achieve mass accuracies of 10 ppm or better, routinely! It has become an integral part of our characterization pipeline for newly synthesized compounds."
University of ViennaDr. Alexander Leitner
"We have used your MassWorks for all our publications of this year (for formula ID), we have found that in most of the cases the (mass) error is about 10 ppm" ( using LC and GC/MS single quads in 13 publications in organocatalysis in 2017-2018 all in peer reviewed journals)
Universidad Autónoma de MadridDr. José Alemán
"With PFTBA calibration performed to each GC/MS run, mass accuracy better than 10mDa can be achieved and Spectral Accuracy of better than 99% can be achieved to help attain reliable formula determination. The mass calibration is stable after 11 days without significantly compromising formula determination. While mass calibration is more susceptible to time related variations, the Spectral Accuracy has been shown to be robust enough for formula determination after 19 days." -Click here to read full presentation
Boehringer IngelheimJ.Mullis,F.Qiu,Y.Wang ASMS2008
"Starting a little bit "speechless" from our side your Presentation helped really changing our mindset, from "unbelieving" into "believer". After this Test with a sample taken from real life with real interferences my colleagues and I fully agree that MassWorks is indeed a powerful tool for advanced GC/MS analysis. We found that MassWorks is really a "must have" product for us..."
Researcher from a large German chemical company
"I have tested and used the MassWorks software since the summer of 2007. The main purpose for me is using its formula ID feature to confirm the GC/MS NIST library search results for unknown chemical substance identification especially when the [NIST] search match values are similar for more than one compounds. The software is relatively simple to use and most times provides the #1 match for our testing chemicals with more than 200 compounds in a variety of chemical groups."
User from a United States Government Environmental Lab
"Determining the elemental composition of pure materials at modest resolution typical of quads has never been routinely practiced – CLIPS brings that task very close to practice for the general user. It is not too much of a stretch to suggest CLIPS will enhance the confidence of TOF-based assignments as well, which may even challenge slower FTMS-based determinations."
User at a large midwest consumer products company