Biomolecules are typically measured as multiply charged ions with MS, requiring post-processing to convert the measured m/z into the original mass through an iterative, typically nonlinear, and often ill-understood process involving trial values for the original mass. Recognizing that MS measures not just single masses but entire distributions of all relevant masses and their abundances, we propose a different approach by eliminating the error-and artifact-prone original mass estimation step from the process altogether while achieving biomolecule identification and relative quantitation through full spectral calibration and analysis. This new approach was tested here with synthetic RNA molecules measured from a higher resolution LC-TOF and compared to unit resolution single quadrupole LC-MS for the identification and relative quantitation of RNA molecules and their modifications. Accurate identification of RNA molecules, their fragments, and modifications are of great scientific and therapeutic importance.
Please find our additional resources below:
- Full Spectral Confirmation of Multiply Charged RNA Molecules and Their Modifications with a Single Quadrupole LC/MS SystemNew York Institute of TechnologyN. Zhang,S. Shi,S. Shang,D. Good,Y.Wang,D.Kuehl ASMS 2021
- 2D-HELS-AA MS Seq: Direct sequencing of tRNA reveals its different isoforms and multiple dynamic base modificationsBioRxivNing Zhang, Shundi Shi, Xuanting Wang, Wenhao Ni, Xiaohong Yuan, Jiachen Duan, Tony Z. Jia, Barney Yoo, Ashley Ziegler, James J. Russo, Wenjia Li, Shenglong Zhang
- SAMMI: A Direct Computational Approach to the Analysis of Multiply Charged Biomolecules and Their Modifications with Electrospray Mass SpectrometryNew York Institute of TechnologyN. Zhang,S. Shi,S. Shang,D. Good,Y.Wang,D.Kuehl ASMS 2019
- Determination of Ligand Number and Distribution in Intact Protein Conjugates with High Mass MALDI-TOF-MSNational Cancer InstituteC. C. Lai; J. A. Kelley, Y. Wang, D. Kuehl
- Quantitative Analysis of Post Translation Modification of Protein Therapeutics at a Subunit Level by Spectral DeconvolutionJohnson & JohnsonM.Gu,K.Sen,A.Mahan,Y.Jmeian,Y.Wang,D.Davis ASMS2015